Contact us for protocols not listed
Faculty Leader: Manish Patankar
Facility Manager: Dagna Sheerar
Tech Notes
- Adding Flow Cytometry to Your BioArrow Protocol
- Biosafety guidelines for cell sorting
- Cell Cycle Analysis
- Compensation Beads
- Doublet Discrimination
- Flow Server Connect Mac
- Flow Server Connect PC
- FlowJo for Antibody Titrations
- Minimizing Aggregates in Samples
- Multicolor Flow from Start to Finish: A Quick Reference Guide
- Rainbow Bead Standardization – Attune
- Rainbow Bead Standardization – Diva
- Regaining Keyboard Control After Diva Locks it Out
- Setup Guide for Running LegendPlex on Attune
- Single Cell Sorting for Transfected Cells
- Sort Sample Prep Guidelines
- Titrating Antibodies for Flow Cytometry
Related Resources
Flow Cytometry Tutorials
Panel Design Resources
A Note on FluoroFinder 2.0: The UWCCC Flow Lab has an institutional license to Academic+ for anyone with an “@wisc.edu” email address. Academic+ includes unlimited access to Intellipanel, and A.I. assisted automated panel design tool (useful for high-parameter flow and spectral panels), a Reagent Inventory Tool, and access to additional trainings and workshops. If you do not currently have access to Academic+ features, please email Dagna Sheerar to request access.
Related Links
- Immunology Wisconsin
- UW Research Cores Directory
- Flow Cytometry Online Appointment Scheduler
- BD Biosciences Spectrum Guide
- Clinical Cytometry Society
- OMIPs (Optimized Multicolor Immunophenotyping Panels), Cytometry Part A
- International Society for the Advancement of Cytometry
- Purdue University Flow Cytometry Laboratories
- OMIP Detailed Spreadsheet (Markers, Clones, Fluors)
- FluoroFinder Panel Builder
- Molecular Probes Handbook
- BioLegend Cell Markers Encyclopedia