Equipment & Pricing

Two ThermoFisher Attune Next Flow Cytometers: “Thunder” and “Lightning”

Location: WIMR 7018D
The Attune NxT is a benchtop analysis cytometer with 4 lasers (488, 405, 561, and 633nm). Capable of collecting up to 16 parameters: 2 scatter and 14 fluorescent, the optical benches are user-configurable to accommodate unusual fluorochromes and unique combinations. These instruments use acoustic focusing to center cells in the stream, allowing for significantly increased acquisition speeds (up to 1000uL per minute) with little to no loss in resolution. They are also equipped with plate auto-samplers for regular and deep-well 96 and 384 well plates.

• Attune Optical Bench Map
• Attune Optical Bench Map – Blank

ThermoFisher Attune NxT V6 Flow Cytometer: “River”

Location: Biotech 2360
The Attune NxT is a benchtop analysis cytometer with 4 lasers (488, 405, 561, and 633nm). Capable of collecting up to 16 parameters: 2 scatter and 14 fluorescent, the optical benches are user-configurable to accommodate unusual fluorochromes and unique combinations. These instruments use acoustic focusing to center cells in the stream, allowing for significantly increased acquisition speeds (up to 1000uL per minute) with little to no loss in resolution. They are also equipped with plate auto-samplers for regular and deep-well 96 and 384 well plates. The V6 model is configured with 6 channels off the violet laser, allowing expanded usage of the available violet-excitable dyes including the SuperBright and Brilliant Violet dye series.
A versatile instrument, the Attune V6 can be used for multi-color immunophenotyping, automated high-throughput screening of samples in plates (i.e., assessment of transfection efficiency), as well as common flow cytometry assays such as cell cycle, apoptosis & cell death, phosphoproteins, mRNA, intracellular cytokines and transfection factors, proliferation, fluorescent proteins, etc.

• Attune V6 Optical Bench Map
• Attune V6 Optical Bench Map – Blank

ThermoFisher Attune Next Flow Cytometer for Analysis of Radiotherapy Samples

Location: WIMR B1334
Managed by the UWCCC Flow Lab and located in the UWCCC SAIRF, this instrument is reserved for the analysis of radiotherapy samples. Users must have completed the Flow Lab’s training program, and Radiation Safety Training in order to use this instrument.
The Attune NxT is a benchtop analysis cytometer with 4 lasers (488, 405, 561, and 633nm). Capable of collecting up to 16 parameters: 2 scatter and 14 fluorescent, the optical benches are user-configurable to accommodate unusual fluorochromes and unique combinations. These instruments use acoustic focusing to center cells in the stream, allowing for significantly increased acquisition speeds (up to 1000uL per minute) with little to no loss in resolution. They are also equipped with plate auto-samplers for regular and deep-well 96 and 384 well plates.

• Attune Optical Bench Map
• Attune Optical Bench Map – Blank

ThermoFisher Attune NxT “Peds”

Location: WIMR 4048
Acquired through generous gifts from the Lion’s Club and the MACC Fund, this instrument is reserved for Pediatric Cancer Research Labs.  Please Contact the Flow Lab for more information.
The Attune NxT is a benchtop analysis cytometer with 4 lasers (488, 405, 561, and 633nm). Capable of collecting up to 16 parameters: 2 scatter and 14 fluorescent, the optical benches are user-configurable to accommodate unusual fluorochromes and unique combinations. These instruments use acoustic focusing to center cells in the stream, allowing for significantly increased acquisition speeds (up to 1000uL per minute) with little to no loss in resolution. They are also equipped with plate auto-samplers for regular and deep-well 96 and 384 well plates.

• Attune Optical Bench Map
• Attune Optical Bench Map – Blank

BD LSR Fortessa

Location: WIMR 7018D

The LSRII is a 5 laser (355nm, 405nm, 488nm, 561nm, and 640nm) 20 parameter (2 scatter and 18 fluorescence) benchtop analysis cytometer.  The optical bench is user configurable for detection of less common fluorochromes and unique combinations.  The Fortessa also has a High-Throughput Sampler for automated sampling of 96 and 384 well plates.

Fortessa Optical Bench Map

Fortessa Optical Bench Map – Blank

ImageStream

Location: WIMR 7018A

The ImageStream MarkII is an imaging cytometer for cells in suspension. It has high resolution CCD cameras instead of photomultiplier tubes for detection and is equipped with 20x, 40x and 60x objectives, six detection channels and two lasers (488nm and 642nm).

ImageStream Optical Bench Map

BD FACSAria with Biosafety Cabinet “Jill” in WIMR 7018B

Best Used For: Higher parameter sorts (6+ colors), sorts that require staff assistance, new users, infrequent sorts, unique sorts (nuclei, bacteria, yeast, cell cycle, and other unusual particles), UV excitable dyes. BSL-2, Limited BSL2+, and NHP SPF

All sort requests require a current Biological Safety Protocol number in which all materials brought for sorting are described and approved by the UW Office of Biological Safety for cell sorting in the Flow Lab.

Sort requests will not be approved without a current and relevant Biological Safety Protocol number.

Cell sorting flow cytometer capable of sorting up to 4 populations simultaneously into tubes, single and multiple cell deposition into 6 – 384 well plates and other custom devices.  Equipped with 5 lasers:  488nm, 405nm, 640nm, 532nm, and 355nm, 16 fluorescence detection channels, and ability to customize emission filters for specific fluorochromes. Contained in custom biological safety cabinet for sorting up to BSL-2 single cell suspensions.

• Aria “Jill” Optical Bench Map

BD FACSAria with Biosafety Cabinet “Jack” in WIMR 7018B

Best Used For: Higher parameter sorts (6+ colors), sorts that require staff assistance, new users, infrequent sorts, unique sorts (nuclei, bacteria, yeast, cell cycle, and other unusual particles). BSL-2, Limited BSL2+, and NHP SPF

All sort requests require a current Biological Safety Protocol number in which all materials brought for sorting are described and approved by the UW Office of Biological Safety for cell sorting in the Flow Lab.

Sort requests will not be approved without a current and relevant Biological Safety Protocol number.

Cell sorting flow cytometer capable of sorting up to 4 populations simultaneously into tubes, single and multiple cell deposition into 6 – 384 well plates and other custom devices. Equipped with 4 lasers: 488nm, 405nm, 640nm, and 561nm, 13 fluorescence detection channels, and ability to customize emission filters for specific fluorochromes.  Contained in custom biological safety cabinet for sorting up to BSL-2 single cell suspensions.

• Aria “Jack” Optical Bench Map

BD FACSAria in BioBubble “Jayne” in Biotech 2360

Best Used For: Higher parameter sorts (6+ colors), sorts that require staff assistance, new users, infrequent sorts, unique sorts (nuclei, bacteria, yeast, cell cycle, and other unusual particles), sorts for downstream Single Cell Genomics, UV excitable dyes. BSL-1 and BSL-2 Only

If sorting yeast or bacteria, please reserve an additional 45 minutes at the end of your reservation for instrument cleaning.

All sort requests require a current Biological Safety Protocol number in which all materials brought for sorting are described and approved by the UW Office of Biological Safety for cell sorting in the Flow Lab.

Sort requests will not be approved without a current and relevant Biological Safety Protocol number.

Installed in March of 2019, this cell sorter is equipped with 5 lasers (488nm, 405nm, 633nm, 561nm, and 355nm) and 18 fluorescence detection channels. This instrument is contained in a BioBubble negative pressure containment unit and is strictly limited to BSL-2 or lower samples only. It is capable of sorting up to 4 populations simultaneously into tubes, single and multiple cell deposition into 6 – 384 well plates and other custom devices.

This Aria has been placed in the UW Biotechnology Center to support Single Cell Genomics pipelines as well as to provide cell sorting to central campus research labs.  Located in the same building as the following cores; Gene Expression Center, DNA Sequencing, Genome Editing & Animal Models, and the Bioinformatics Resource Center, this instrument is uniquely positioned to provide highly purified cell subpopulations and single cell per well deposition into up to 384 well plates for downstream genomics assays.

• Aria “Jayne” Optical Bench Map

Scheduling

The Flow Lab may adjust requested start times for sort appointments to optimize available instrument time.

Biosafety and Cell Sorting

Anything sorted in the Flow Lab MUST be described on your Lab’s Biological safety protocol.  Cell sorting creates aerosols and additional biological safety precautions are required.  The Flow Lab is able to sort samples of BSL-2 and below.

All sort requests require a current Biological Safety Protocol number in which all materials brought for sorting are described and approved by the UW Office of Biological Safety for cell sorting in the Flow Lab.

Sort requests will not be approved without a current and relevant Biological Safety Protocol number.

• Adding Flow Cytometry to your Bio ARROW Protocol

Flow Lab Hood Certification Dates

• • Baker BioProtect Aria “Jill” Cell Sorter (7018B WIMR), SN 120708, Cert. Date: 11/28/24
  • Baker BioProtect Aria “Jack” Cell Sorter (7018B WIMR), SN 108121, Cert. Date: 11/28/24
  • Baker BCC300AMS Sony MA900 Cell Sorter (7016C WIMR), SN 138210, Cert Date: 11/28/24
  • BioBubble Aria “Jayne” Cell Sorter (2360 Biotech), SN PU2525-0159, Cert. Date: 03/11/24
  • 4′ Baker SteriGuardIII Advance (7018E WIMR), SN 93707, Cert. Date: 11/27/24
  • 6′ Baler SteroGuardIII Advance (7018E WIMR), SN 95206, Cert. Date: 11/27/24
  • 4′ Baker SteriGuardIII Advance (2360 Biotech), SN 90115, Cert. Date: 03/11/24

The Sony MA900 Multi-Application Cell Sorter with sample and collection devices.

Best Used For: Fluorescent Proteins (GFP, mCherry, tdTomato, etc.), lower parameter sorts (1-8 colors), End-User Operation (self-sorting), After-hours and Weekend Self-Sorting. BSL-1 and BSL-2 Only.

All sort requests require a current Biological Safety Protocol number in which all materials brought for sorting are described and approved by the UW Office of Biological Safety for cell sorting in the Flow Lab.

Sort requests will not be approved without a current and relevant Biological Safety Protocol number.

Location: WIMR 7018C

The Sony MA900 is a highly automated cell sorter allowing for end-user self-operation and we will be training researchers to operate this instrument independently.  This will allow for more flexible scheduling, and will be available to trained researchers after-hours and on weekends to accommodate unusual time points, long pre-processing workflows, and clinical research samples with later arrival times.

Training is composed of 2 sessions:

• AM Session:  9:30-11:30am – Start-up & QC Training
• PM Session: 1:30-3:30pm – Cell Sorting & Shutdown Training.  Customers should bring stained cells for sorting. Training will include setting up the sort, adjusting sensitivity, compensation, sorting, saving a template, rigor and reproducibility considerations, and instrument shutdown.
• Staff is available for additional training/assistance as needed.  Please email the Flow Lab at uwflow@uwcarbone.wisc.edu if you need staff assistance during a self-run reservation during set-up, or for a new assay.

The MA900 is capable of sorting up to 4 populations simultaneously into tubes, single and multiple cell deposition into 6 – 384 well plates and other custom devices, including PCR plates.  Equipped with 4 lasers arranged in co-linear pairs:  488nm & 561nm, and, 405nm and 634nm, 12 fluorescence detection channels, and ability to customize emission filters for specific fluorochromes. Contained in custom biological safety cabinet for sorting up to BSL-2 single cell suspensions.

Sony MA900 Optical Bench

Sony MA900 Fluorochrome and Filter Guide

Sony MA900 Manufacturer Webpage

Location: WIMR 7016

The Countess 3 FL Automated Cell Counter is a benchtop assay platform equipped with state-of-the-art optics, fully automated focus and lighting capability, and image analysis software driven by a neural network-based machine-learning algorithm, for rapid assessment of primary and immortalized cell lines. The Countess 3 FL counter is capable of brightfield or fluorescence illumination with three-channel flexibility (brightfield and two optional fluorescence channels) enabling researchers to count cells, monitor fluorescent protein expression, gain insights to apoptotic processes, and measure cell viability.

• Accurate—eliminates the subjectivity of manual cell counting and user-to-user variability
• Flexible—accepts up to two EVOS light cubes, user selectable from fifteen fluorescent channels
• Fast—counts live and dead cells, measures viability, reports average cell size, and saves data with a single touch, in about 20 seconds
• Convenient—requires no cleaning or routine maintenance; high-resolution capacitive touchscreen and simple user interface provide quick startup and requires minimal training

Filter cubes available in Flow Lab: GFP, PI/RFP/mCherry, DAPI

Counting Chambers available at cost from Flow Lab or order from Shop@UW: Cat# C10228 (Box of 50), Cat# C10312 (Box of 500)

Picture and description from ThermoFisher website, more info here: Countess 3FL Automated Cell Counter

Location: WIMR 7016

The Nexcelom Cellometer Auto T4 Cell Viability Counter is a PC-based instrument for one-step 4x bright field image capture and analysis. This state-of-the-art cell counter performs count, size, and Trypan Blue viability analyses that are 10x faster and more accurate than manual counting. This instrument is ideal for cell lines and purified primary cells, including MCF-7 breast cancer cells, PC3 prostate cancer cells, and 1600 other cell types, due to its advanced software that assesses clumpy cells and omits debris. This is a slide based cell counter and requires Nexcelom counting slides. There are 2 tests per slide and the slides can be bought individually or in packs of 75 from the Flow Lab.

Offline data analysis computers are available to laboratory users only and are located at the Clinical Science Center in room K4/532, and at the Biotech Center in room 2360. Computers are loaded with a variety of analysis packages including FlowJo, FCS Express, SpectroFlo, ModFit, WinList, and IDEAS ImageStream analysis software, Microsoft Office, and Adobe Illustrator.

There is no charge for the use of these computers and are for the exclusive use for the analysis of data generated at this laboratory. Computers are available on a first-come, first-served walkup basis for users of the facility. Contact Flow Lab staff for access to these rooms.