Our Mission:
The mission of the University of Wisconsin Carbone Cancer Center Flow Lab is to maintain a cutting-edge facility that provides researchers the technical and educational support for fluorescence-based single cell analysis and isolation to further the characterization and understanding of cellular function, biomarkers, pathology, and treatment in basic, translational, and clinical research projects.
The Flow Lab was established in 1988 as a resource for the University of Wisconsin–Madison campus. The facility maintains two laboratories: the main facility located in the Wisconsin Institutes for Medical Research (WIMR) a full service satellite facility in the UW Biotechnology Center.
COVID Policies
Masks are required at all times in the UW Biotechnology Building for those accessing our lab in 2360 Biotech.
Masks are currently required in all spaces of WIMR where patients may be present (lobby, elevators, Floors 1 and 2). Masks are not required (but certainly welcome) in research spaces for persons who have been vaccinated. Social distancing should continue to be practiced at all times.
Flow Lab Holiday Hours 2021
Data Analysis Software
The Flow Lab hosts site licenses for both FlowJo and FCS Express flow cytometry data analysis software packages.
Locations
Flow Cytometry’s labs are located in the Wisconsin Institute for Medical Research (WIMR) and the UW Biotechnology Center.
Contact Us
Faculty Leader: Manish Patankar
Facility Manager: Dagna Sheerar (Certified Cytometrist)
Facility Staff: Kathryn Fox (Certified Cytometrist), Lauren Nettenstrom (Certified Cytometrist), Zach Stenerson, Alex Henkel, Kyle Christie, and Ofelia Lapacek
Email: uwflow@uwcarbone.wisc.edu
WIMR 7016 Phone: 608-263-0313
Biotech 2360 Phone: 608-265-5547
Acknowledgments
Any published research which received funding from the UWCCC Cancer Center Support Grant (CCSG), including the use of UWCCC Shared Resources to generate or analyze data or conduct clinical trials, must reference the UWCCC. See Acknowledging UWCCC in Publications, Posters, and Presentations.
If you have the generated data on the following instruments, you must acknowledge the following NIH Shared Instrumentation Grants:
- BD FACS AriaII BSL-2 Cell Sorter (“Jill”)
- Grant #: 1S10RR025483-01
- Project Title: Multi-color Benchtop Flow Cytometer
- BD LSR Fortessa
- Grant#: 1S10OD018202-01
- Project Title: Special BD LSR Fortessa
- Cytek Aurora Spectral Cytometer
- Grant#: 1S10OD025225-01
- Project Title: BD FACSymphony High Parameter Flow Cytometer
If you have existing publications that have used either of the instruments listed above, but have not cited the grant, please update these publications to include the relevant grant acknowledgement(s).
Instructions for acknowledging grants on existing publications.
Connect with Flow
Flow Cytometry's Latest Posts
Update to Flow Lab Distancing Guidelines
With the new COVID testing requirements, we will be able to operate both sorters at WIMR and will be removing the social distancing restrictions for the analysis instrumentation beginning February 8th. We ask that users …
February 10, 2021FCS Express Resources from Home
DeNovo Software and the UWCCC Flow Cytometry Lab are teaming up to provide support for FCS Express flow cytometry data analysis software remotely. Whether you’re just getting started or need some help with some of …
November 13, 2020Webinar – Apr 30, 2020 @ 2pm Central: Sorting Hazardous Materials on the MACSQuant Tyto
Join the UWCCC Flow Lab on Thursday, April 30 at 2pm Central for a webinar on sorting hazardous materials with the Miltenyi MACSQuant Tyto cell sorting cytometer. This is a closed system with a very, …
April 27, 2020- More Flow Cytometry Lab posts
Flow Resources
Using the Facility
The Flow Cytometry Lab has two locations: Wisconsin Institutes for Medical Research (WIMR) Room 7016 (laboratory) and 7003 (office), and McArdle Building Room 118.
Equipment & Pricing
Information about training, equipment, and pricing for the UWCCC Flow Cytometry Lab.
Tech Notes & Related Resources
Flow Cytometry protocols, technical notes, and related resources.