UWCCC Flow Lab Research Reboot
The Flow Lab is currently operating under restricted access Research Reboot operational guidelines. In order to access the Flow Lab, you must list the Flow Lab in your lab’s Phase 1 plan, have been approved, and staff requesting access must have completed the COVID-19 Training for On-Site Workers.
Please see our COVID-19 Flow Resource Page and the links below for more information.
UWCCC Flow Lab Operations Plan
SOP for Accessing Flow Lab at WIMR
SOP for Accessing Flow Lab at UW Biotechnology Center
Cell Sorting Sample Form – Required for Sorting
Instrument Training and Staff Assisted Appointments are now available via remote training/assisted using Microsoft Teams. Please contact the Flow Lab at uwflow@uwcarbone.wisc.edu for more information.
The computer lab in K4/519 Clinical Sciences Center remains closed. Please contact the Flow Lab at uwflow@uwcarbone.wisc.edu if you need access to any of the data analysis software packages available in the computer lab.
We are asking that customers with scheduled sorts wait to enter the Flow Lab until contacted by the Flow Lab staff to ensure that the instrument and operator are ready to receive samples.
We are also asking that customers using the analysis cytometers refrain from requesting assistance of the sort operator and instead contact the members of the Flow Lab working from home via uwflow@uwcarbone.wisc.edu
Customers are required to disinfect all surfaces used during their appointments BEFORE and AFTER use with 70% EtOH. See SOP for Accessing Flow Lab at WIMR
The above requests are to allow us to provide the best possible services to those of you with approved essential research while adhering to social distancing guidelines. We thank you very much for your understanding and are looking forward to being able to work closely with all of you again in the, hopefully, near future.
Please check this page and the Flow Lab’s COVID-19 page for updates as the situation develops.
Thank you for your patience during this challenging time,
The Flow Lab Staff
https://covid19.wisc.edu
The mission of the University of Wisconsin Carbone Cancer Center Flow Lab is to maintain a cutting-edge facility that provides researchers the technical and educational support for fluorescence-based single cell analysis and isolation to further the characterization and understanding of cellular function, biomarkers, pathology and treatment in basic, translational, and clinical research projects.
On this page:
The Flow Lab was established in 1988 as a resource for the University of Wisconsin–Madison campus. The facility maintains two laboratories: the main facility located in the Wisconsin Institutes for Medical Research (WIMR) a full service satellite facility in the UW Biotechnology Center.
Data Analysis Software
The Flow Lab hosts site licenses for both FlowJo and FCS Express flow cytometry data analysis software packages.
Locations
Flow Cytometry’s labs are located in the Wisconsin Institute for Medical Research (WIMR) and the UW Biotechnology Center.
Contact Us
Faculty Leader: Manish Patankar
Facility Manager: Dagna Sheerar (Certified Cytometrist)
Facility Staff: Kathryn Fox (Certified Cytometrist), Lauren Nettenstrom (Certified Cytometrist), Zach Stenerson, Alex Henkel, Abigale Bleil
Email: uwflow@uwcarbone.wisc.edu
WIMR 7016 Phone: 608-263-0313
Biotech 2360 Phone: 608-265-5547
Acknowledgments
Any published research which received funding from the UWCCC Cancer Center Support Grant (CCSG), including the use of UWCCC Shared Resources to generate or analyze data or conduct clinical trials, must reference the UWCCC. See Acknowledging UWCCC in Publications, Posters, and Presentations.
If you have the generated data on the following instruments, you must acknowledge the following NIH Shared Instrumentation Grants:
- BD FACS AriaII BSL-2 Cell Sorter (“Jill”)
- Grant #: 1S10RR025483-01
- Project Title: Multi-color Benchtop Flow Cytometer
- BD LSR Fortessa
- Grant#: 1S100OD018202-01
- Project Title: Special BD LSR Fortessa
If you have existing publications that have used either of the instruments listed above, but have not cited the grant, please update these publications to include the relevant grant acknowledgement(s).
Instructions for acknowledging grants on existing publications.
Connect with Flow
Flow Cytometry's Latest Posts
Webinar – Apr 30, 2020 @ 2pm Central: Sorting Hazardous Materials on the MACSQuant Tyto
Join the UWCCC Flow Lab on Thursday, April 30 at 2pm Central for a webinar on sorting hazardous materials with the Miltenyi MACSQuant Tyto cell sorting cytometer. This is a closed system with a very, …
April 27, 2020Upcoming Webinars
The flow lab will be hosting several webinars focusing on flow cytometry data analysis over the next few weeks. Thursday, April 16th: 10 am “The FCS Express Difference.” This webinar includes an overview of the …
April 13, 2020New Instrumentation, Computer Lab, Old Instrumentation
The UWCCC Flow Lab has some exciting changes happening soon! The Aurora Spectral Cytometer will be delivered on Wednesday, January 22, 2020. This is a 5-Laser, 67-Channel spectral cytometer, which collects a “fluorescence signature” for …
January 16, 2020- More Flow Cytometry Lab posts
Flow Resources
Using the Facility
The Flow Cytometry Lab has two locations: Wisconsin Institutes for Medical Research (WIMR) Room 7016 (laboratory) and 7003 (office), and McArdle Building Room 118.
Equipment & Pricing
Information about training, equipment, and pricing for the UWCCC Flow Cytometry Lab.
Tech Notes & Related Resources
Flow Cytometry protocols, technical notes, and related resources.