Our Mission:
The mission of the University of Wisconsin Carbone Cancer Center Flow Lab is to maintain a cutting-edge facility that provides researchers the technical and educational support for fluorescence-based single cell analysis and isolation to further the characterization and understanding of cellular function, biomarkers, pathology, and treatment in basic, translational, and clinical research projects.
The Flow Lab was established in 1988 as a resource for the University of Wisconsin–Madison campus. The facility maintains two laboratories: the main facility located in the Wisconsin Institutes for Medical Research (WIMR) a full service satellite facility in the UW Biotechnology Center.
COVID Policies
Masks and BSL-2 PPE are required at all times in both the WIMR and UW Biotechnology Center in which our labs are located. Social distancing should continue to be practiced at all times.
UW COVID-19 Response: https://covidresponse.wisc.edu
Flow Lab Holiday Hours 2021
Data Analysis Software
The Flow Lab hosts site licenses for both FlowJo and FCS Express flow cytometry data analysis software packages.
Locations
Flow Cytometry’s labs are located in the Wisconsin Institute for Medical Research (WIMR) and the UW Biotechnology Center.
Contact Us
Faculty Leader: Manish Patankar
Facility Manager: Dagna Sheerar (Certified Cytometrist)
Facility Staff: Kathryn Fox (Certified Cytometrist), Lauren Nettenstrom (Certified Cytometrist), Zach Stenerson, Alex Henkel, Kyle Christie, and Ofelia Lapacek
Email: uwflow@uwcarbone.wisc.edu
WIMR 7016 Phone: 608-263-0313
Biotech 2360 Phone: 608-265-5547
Acknowledgments
Any published research which received funding from the UWCCC Cancer Center Support Grant (CCSG), including the use of UWCCC Shared Resources to generate or analyze data or conduct clinical trials, must reference the UWCCC. See Acknowledging UWCCC in Publications, Posters, and Presentations.
If you have the generated data on the following instruments, you must acknowledge the following NIH Shared Instrumentation Grants:
- BD FACS AriaII BSL-2 Cell Sorter (“Jill”)
- Grant #: 1S10RR025483-01
- Project Title: Multi-color Benchtop Flow Cytometer
- BD LSR Fortessa
- Grant#: 1S10OD018202-01
- Project Title: Special BD LSR Fortessa
- Cytek Aurora Spectral Cytometer
- Grant#: 1S10OD025225-01
- Project Title: BD FACSymphony High Parameter Flow Cytometer
If you have existing publications that have used either of the instruments listed above, but have not cited the grant, please update these publications to include the relevant grant acknowledgement(s).
Instructions for acknowledging grants on existing publications.
Connect with Flow
Flow Cytometry's Latest Posts
Aurora User Group Seminar: Spectral Unmixing and Compensation
Learn about Spectral Cytometry with the UWCCC Flow Cytometry Laboratory! Thursday January 27th, 2022 from 10am to 11am. This presentation is part of our Aurora User Group and anyone is welcome to attend. Kathryn Fox …
January 20, 2022On-Demand Webinar: Creative Funding Options for SRLs: from Crowd Funding to Philanthropy
Link to Webinar at CYTO U: https://learning.isac-net.org/products/creative-funding-options-for-srls-from-crowd-funding-to-philanthropy Webinar Summary: There is often a lot of pressure on SRL managers to run a partial- or full-cost-recovery core facility which presents many challenges. Identifying and securing external …
December 9, 2021New Instrumentation in the Flow Lab!
The Sony MA900, or Multi-Application Cell Sorter was installed in the Flow Lab in WIMR 7016 in late September 2021. The Flow Lab Staff are working to make this instrument available to researchers within the next …
October 13, 2021- More Flow Cytometry Lab posts
Flow Resources
Using the Facility
The Flow Cytometry Lab has two locations: Wisconsin Institutes for Medical Research (WIMR) Room 7016 (laboratory) and 7003 (office), and McArdle Building Room 118.
Equipment & Pricing
Information about training, equipment, and pricing for the UWCCC Flow Cytometry Lab.
Tech Notes & Related Resources
Flow Cytometry protocols, technical notes, and related resources.