Our Mission:
The mission of the University of Wisconsin Carbone Cancer Center Flow Lab is to maintain a cutting-edge facility that provides researchers the technical and educational support for fluorescence-based single cell analysis and isolation to further the characterization and understanding of cellular function, biomarkers, pathology, and treatment in basic, translational, and clinical research projects.
The Flow Lab was established in 1988 as a resource for the University of Wisconsin–Madison campus. The facility maintains two laboratories: the main facility located in the Wisconsin Institutes for Medical Research (WIMR), and a full-service satellite facility in the UW Biotechnology Center.
Data Analysis Software
The Flow Lab hosts site licenses for both FlowJo and FCS Express flow cytometry data analysis software packages.
Locations
Flow Cytometry’s labs are located in the Wisconsin Institute for Medical Research (WIMR) and the UW Biotechnology Center.
Contact Us
Faculty Leader: Manish Patankar
Facility Manager: Dagna Sheerar (Certified Cytometrist)
Facility Staff: Kathryn Fox (Certified Cytometrist), Zach Stenerson, Kyle Christie, Ofelia Lapacek, and Tenley Baumann.
Email: uwflow@uwcarbone.wisc.edu
WIMR 7016 Phone: 608-263-0313
Biotech 2360 Phone: 608-265-5547
Acknowledgments & Authorship
Guidelines for Appropriate Recognition of Shared Resource Laboratories (Cytometry Part A, 2023)
Authorship Guidelines from the Association of Biomolecular Resources Facilities
Any published research which received funding from the UWCCC Cancer Center Support Grant (CCSG), including the use of UWCCC Shared Resources to generate or analyze data or conduct clinical trials, must reference the UWCCC. See Acknowledging UWCCC in Publications, Posters, and Presentations. Acknowledgements and Authorship are critical to continued support of all Shared Resource Laboratories.
Acknowledge “UWCCC Flow Cytometry Laboratory” for use of all equipment housed in the Flow Lab.
Please consider Flow Lab staff Authorship for the following support:
- Experimental Design & Troubleshooting
- Panel Design & Troubleshooting
- Data Analysis: Troubleshooting, Methodology, Data Analysis Performed by Flow Lab staff
- Manuscript Review & Editing
- Figure Generation & Editing
- For anything you would normally consider worthy of an Authorship Credit for your lab members and colleagues
Sample Acknowledgment:
“The author(s) thank the University of Wisconsin Carbone Cancer Center Flow Cytometry Laboratory, supported by P30 CA014520, for use of its facilities and services.”
If you have the generated data on the following instruments, you must acknowledge the following NIH Shared Instrumentation Grants:
- BD FACS AriaII BSL-2 Cell Sorter, “Jill”
- Grant #: 1S10RR025483-01
- Project Title: Multi-color Benchtop Flow Cytometer
- BD LSR Fortessa
- Grant#: 1S10OD018202-01
- Project Title: Special BD LSR Fortessa
- Cytek Aurora Spectral Cytometer, “Pinky”
- Grant#: 1S10OD025225-01
- Project Title: BD FACSymphony High Parameter Flow Cytometer
If you have existing publications that have used either of the instruments listed above, but have not cited the grant, please update these publications to include the relevant grant acknowledgement(s).
Instructions for acknowledging grants on existing publications.
Connect with Flow
Flow Cytometry's Latest Posts
Flow Cytometry Panel Design and Educational Support with FluoroFinder, November 7, 2024
Please join the UWCCC Flow Lab on November 7, 2024 at 1pm in WIMR 7001A for a seminar on Panel Design for Flow Cytometry. Ryan Metzler from FluoroFinder will present their machine learning supported panel …
October 18, 2024Webinar: Sept 25, 2024, 12pm Central, Feasibility of an Immune Monitoring Panel with Drop-In Channels for Spectral Flow Cytometry
Please join us for a webinar focusing on technical challenges for reference control selection and autofluorescence extraction in spectral cytometry. Presented by Jolly Jensen PhD, Senior Scientist at Amgen. Dr. Jensen will focus specifically on …
September 19, 2024Seminar – September 26, 2024: Tips and Tricks for High Parameter Flow and Spectral Cytometry & Multiplexing using Luminex Technology
The UWCCC Flow Cytometry Laboratory will be hosting a seminar presented by ThermoFisher staff scientist Seddon Thomas, PhD, and Technical Applications Specialist Kathryn Lubinski, focusing on tips and tricks for conventional and spectral cytometry including …
September 17, 2024- More Flow Cytometry Lab posts
Flow Resources
Using the Facility
The Flow Cytometry Lab has two locations: Wisconsin Institutes for Medical Research (WIMR) Room 7016 (laboratory) and 7003 (office), and McArdle Building Room 118.
Equipment & Pricing
Information about training, equipment, and pricing for the UWCCC Flow Cytometry Lab.
Tech Notes & Related Resources
Flow Cytometry protocols, technical notes, and related resources.