Cell Sorting Survey:
https://uwmadison.co1.qualtrics.com/jfe/form/SV_9GEOQ0kW75OaWX4
Please take five minutes to complete a short survey of current and future cell sorting needs. Data from this survey will assist the Flow Lab in choosing cell sorters that will meet the needs of researchers for several years to come, and may be used in instrument grant applications. We greatly appreciate you taking the time to take this survey.
Our Mission:
The mission of the University of Wisconsin Carbone Cancer Center Flow Lab is to maintain a cutting-edge facility that provides researchers the technical and educational support for fluorescence-based single cell analysis and isolation to further the characterization and understanding of cellular function, biomarkers, pathology, and treatment in basic, translational, and clinical research projects.
The Flow Lab was established in 1988 as a resource for the University of Wisconsin–Madison campus. The facility maintains two laboratories: the main facility located in the Wisconsin Institutes for Medical Research (WIMR) a full service satellite facility in the UW Biotechnology Center.
COVID Policies
Effective March 12, 2022, masks will no longer be required in indoor settings on UW-Madison campus.
Faculty and staff are still required to wear masks where patients and their families are likely to be present. This includes WIMR lobby, central elevators, and basement. Masks are also required in places with regular traffic from patients and visitors in the CSC and UW Hospital, such as main entrances, cafeterias, and elevators. If you are uncertain as to whether or not a mask should be worn in a certain area, continue to wear a mask in that area.
UW COVID-19 Response: https://covidresponse.wisc.edu
UW Health Masking Guidance: https://uconnect.wisc.edu/inside-uw-health/news/2022/march/name-182042-en.news
UW COVID-19 Response: https://covidresponse.wisc.edu
Data Analysis Software
The Flow Lab hosts site licenses for both FlowJo and FCS Express flow cytometry data analysis software packages.
Locations
Flow Cytometry’s labs are located in the Wisconsin Institute for Medical Research (WIMR) and the UW Biotechnology Center.
Contact Us
Faculty Leader: Manish Patankar
Facility Manager: Dagna Sheerar (Certified Cytometrist)
Facility Staff: Kathryn Fox (Certified Cytometrist), Zach Stenerson, Alex Henkel, Kyle Christie, Ofelia Lapacek, and Tenley Baumann.
Email: uwflow@uwcarbone.wisc.edu
WIMR 7016 Phone: 608-263-0313
Biotech 2360 Phone: 608-265-5547
Acknowledgments & Authorship
Guidelines for Appropriate Recognition of Shared Resource Laboratories (Cytometry Part A, 2023)
Any published research which received funding from the UWCCC Cancer Center Support Grant (CCSG), including the use of UWCCC Shared Resources to generate or analyze data or conduct clinical trials, must reference the UWCCC. See Acknowledging UWCCC in Publications, Posters, and Presentations. Acknowledgements and Authorship are critical to continued support of all Shared Resource Laboratories.
Acknowledge “UWCCC Flow Cytometry Laboratory” for use of all equipment housed in the Flow Lab.
Please consider Flow Lab staff Authorship for the following support:
- Experimental Design & Troubleshooting
- Panel Design & Troubleshooting
- Data Analysis: Troubleshooting, Methodology, Data Analysis Performed by Flow Lab staff
- Manuscript Review & Editing
- Figure Generation & Editing
- For anything you would normally consider worthy of an Authorship Credit for your lab members and colleagues
Sample Acknowledgment:
“The author(s) thank the University of Wisconsin Carbone Cancer Center Flow Cytometry Laboratory, supported by P30 CA014520, for use of its facilities and services.”
If you have the generated data on the following instruments, you must acknowledge the following NIH Shared Instrumentation Grants:
- BD FACS AriaII BSL-2 Cell Sorter (“Jill”)
- Grant #: 1S10RR025483-01
- Project Title: Multi-color Benchtop Flow Cytometer
- BD LSR Fortessa
- Grant#: 1S10OD018202-01
- Project Title: Special BD LSR Fortessa
- Cytek Aurora Spectral Cytometer
- Grant#: 1S10OD025225-01
- Project Title: BD FACSymphony High Parameter Flow Cytometer
If you have existing publications that have used either of the instruments listed above, but have not cited the grant, please update these publications to include the relevant grant acknowledgement(s).
Instructions for acknowledging grants on existing publications.
Connect with Flow
Flow Cytometry's Latest Posts
Intro to FCS Express Workshop: April 11, 2023
Intro to FCS Express Workshop 2pm Tuesday, April 11, 2023 in WIMR 5001A Contact Alex Henkel at amhenkel@wisc.edu for more information.
March 31, 2023Flow Lab Instrument Demo: Bio-Rad ZE5, May 2-5, 2023
The Flow Lab will have Bio-Rad’s ZE5 Cell Analyzer in the Flow Lab the first week of May, 2023, with an informational seminar on Tuesday, May 2, 2023 at 10am in WIMR 7001A. No registration …
March 31, 202345th Annual Course in Cytometry Sponsorship Prospectus
45th Annual Course in Cytometry Sponsor Prospectus
February 16, 2022- More Flow Cytometry Lab posts
Flow Resources
Using the Facility
The Flow Cytometry Lab has two locations: Wisconsin Institutes for Medical Research (WIMR) Room 7016 (laboratory) and 7003 (office), and McArdle Building Room 118.
Equipment & Pricing
Information about training, equipment, and pricing for the UWCCC Flow Cytometry Lab.
Tech Notes & Related Resources
Flow Cytometry protocols, technical notes, and related resources.