Flow Cytometry Lab

Join the Flow Lab on March 12, 2024 at 11am in WIMR 7001A for a seminar on cleaning up single cell suspensions for cell sorting, single cell genomics and other assays.  Using a unique levitation technology, the Levitas instruments can remove debris to help improve sample quality for a host of down stream assays.  Please see the attached flyer for the QR code to register for this seminar.  We will have an on site demo of this technology in early April 2024.  Date for demo will likely be announced at the seminar and watch for follow up communications to sign up for the demo and see how you can improve your sample quality.

More information about Levitas Technology.

Levitas Seminar Flyer -Wisc 2.8.24 Final pdf

The Flow Lab is hosting a seminar presented by Rebecca Bultema from Nodexus, focusing on their NX One single cell sorter.  This is a benchtop sorter for deposition of single cells into 96 well plates specifically designed for cell line development, selection of GFP+ populations for CRISPR workflows, enrichment, liquid reagent dispensing into plates, and some fluorescent based phenotyping capabilities.  Please feel free to join us on February 29th at 11am in WIMR 5001A.

Please join us on Tuesday, January 30 at 10:30am in WIMR 7001A for the upcoming live demo webinar on how to use the FluoroFinder platform, an essential tool for building flow cytometry panels and designing fluorescent experiments. 

As a researcher at University of Wisconsin -Madison, you have access to premium FluoroFinder features such as our fully integrated flow cytometry training course and our automated panel design software, IntelliPanel. Join the webinar to learn how to fully take advantage of the platform or email us at support@fluorofinder.com with any questions. 

To sign up for a FluoroFinder account, visit:   https://app.fluorofinder.com/users/sign_up 

Link to Seminar Flyer: UW-Madison Training – 30JAN24

Intro to FCS Express Workshop

2pm Tuesday, April 11, 2023 in WIMR 5001A

Contact Alex Henkel at amhenkel@wisc.edu for more information.

The Flow Lab will have Bio-Rad’s ZE5 Cell Analyzer in the Flow Lab the first week of May, 2023, with an informational seminar on Tuesday, May 2, 2023 at 10am in WIMR 7001A.  No registration necessary to attend the seminar.  Please contact Dagna Sheerar at dsheerar@wisc.edu if you would like to demo the instrument and/or run some of your own samples.

ZE5 Features:

• 5 Lasers
• 27 Fluorescence Channels
• Tube & Plate AutoSampling (96 & 284 well)
• High-Throughput
  • 50-70K events per second for mammalian cells
  • 96-well plate in 15 minutes
  • 384-well plate in 50 minutes
• Small Particle Detector for Microparticles, Extracellular Vesicles, Exosomes

45th Annual Course in Cytometry Sponsor Prospectus

The University of Wisconsin Carbone Cancer Center Flow Cytometry Laboratory, in partnership with the University of Wisconsin Biotechnology Center, UW Carbone Cancer Center, and Cytometry Educational Associates, Inc., is excited to announce that  registration for the 45th Annual Course in Cytometry is open!

Join us in person from June 18-23, 2022 on the University of Wisconsin – Madison campus for a week of intense and fun filled Cytometry education. With a low instructor to attendee ratio, registrants will have access to instructors and benefit from an intimate learning environment.

The Annual Course in Cytometry is an opportunity for experts and beginners in Cytometry from around the world to increase their knowledge of Cytometry through intimate instruction by recognized leaders in the Cytometry community. With the exception of the 2020 and 2021 pandemic years it has been held every year since 1977. Over the course of six days, approximately 40 instructors and 90 students participate in lectures, including a Keynote Speaker, labs, and networking events. Students have access to over 20 lectures, as well as their choice of 5 of 15 laboratory sessions. We couldn’t do this without the support of our sponsors, who are highlighted in a vendor night each year.

Attendees traditionally come from varied backgrounds including; Core and Shared Resource Lab staff, managers, and directors, Graduate Students, Post-Doctoral Candidates, Scientists, Researchers, and Technicians from both Academia and Industry.

The 45th Annual Course will be hosted by the UWCCC Flow Lab and physically based in the UW Biotechnology Center, a nationally recognized center for biotechnology research housing 6 core labs. In addition to the lectures and labs, we will be hosting a Banquet Dinner at the Vintage Brewery Company in nearby Sauk City, Wisconsin, and offering a trip to the Olbrich Botanical Gardens during the free afternoon on Wednesday, June 22, 2022.

Lecture topics include: Cytometry Basics, Fluorescence in Cytometry, Fluorescent Proteins in Cytometry, Acquisition Basics, Data Analysis Basics, Proliferation Analysis, Intracellular Cytometry, Basic Sorting Strategies, Panel Design and Compensation, Immunology Basics, Multicolor Immunophenotyping, Rigor & Reproducibility in Cytometry, Spectral Cytometry, Single Cell Genomics, Cytometry of Apoptosis, Tracking Cell Trafficking and Function, Image Cytometry, Monitoring Immune Function by Flow Cytometry, Flow Cytometry Instrument Innovations, and Cytometry of Small Particles.

Laboratory topics include: Build a Flow Cytometer, Cell Sorting, Spectral Cytometry, Image Cytometry, Data Acquisition, Panel Design, Cell Function, Proliferation and Apoptosis, High-Dimensional Data Analysis, A Typical Day in the Cytometry Lab, mRNA and Protein Expression, Cytometry Analysis of Extra Cellular Vesicles, Multi-Color Immunophenotyping, Single Cell Genomics, Analysis and Sorting of Complex Tissues.