Attune Map 3-15-24
Optical bench map of the ThermoFisher Attune, with fluorochromes listed
File: Thunder-Lightning-PEDS-SAIRF-Attune-Map.pdfOptical bench map of the ThermoFisher Attune, with fluorochromes listed
File: Thunder-Lightning-PEDS-SAIRF-Attune-Map.pdfOptical bench map of the BD LSR Fortessa, blank for filling in by hand
File: Fortessa-BLANK-Map.pdfOptical bench map of the BD LSR Fortessa, with fluorochromes listed
File: Fortessa-Map.pdfJoin the Flow Lab on March 12, 2024 at 11am in WIMR 7001A for a seminar on cleaning up single cell suspensions for cell sorting, single cell genomics and other assays. Using a unique levitation technology, the Levitas instruments can remove debris to help improve sample quality for a host of down stream assays. Please see the attached flyer for the QR code to register for this seminar. We will have an on site demo of this technology in early April 2024. Date for demo will likely be announced at the seminar and watch for follow up communications to sign up for the demo and see how you can improve your sample quality.
More information about Levitas Technology.
The Flow Lab is hosting a seminar presented by Rebecca Bultema from Nodexus, focusing on their NX One single cell sorter. This is a benchtop sorter for deposition of single cells into 96 well plates specifically designed for cell line development, selection of GFP+ populations for CRISPR workflows, enrichment, liquid reagent dispensing into plates, and some fluorescent based phenotyping capabilities. Please feel free to join us on February 29th at 11am in WIMR 5001A.
Please join us on Tuesday, January 30 at 10:30am in WIMR 7001A for the upcoming live demo webinar on how to use the FluoroFinder platform, an essential tool for building flow cytometry panels and designing fluorescent experiments.
As a researcher at University of Wisconsin -Madison, you have access to premium FluoroFinder features such as our fully integrated flow cytometry training course and our automated panel design software, IntelliPanel. Join the webinar to learn how to fully take advantage of the platform or email us at support@fluorofinder.com with any questions.
To sign up for a FluoroFinder account, visit: https://app.fluorofinder.com/users/sign_up
Link to Seminar Flyer: UW-Madison Training – 30JAN24
Contact Alex Henkel at amhenkel@wisc.edu for more information.
The Flow Lab will have Bio-Rad’s ZE5 Cell Analyzer in the Flow Lab the first week of May, 2023, with an informational seminar on Tuesday, May 2, 2023 at 10am in WIMR 7001A. No registration necessary to attend the seminar. Please contact Dagna Sheerar at dsheerar@wisc.edu if you would like to demo the instrument and/or run some of your own samples.
ZE5 Features:
This document walks through the general steps for setting up the Attune to run a BioLegend LegendPlex assay on the Attune NxT. Authored by Kathryn Fox of the UWCCC Flow Lab with input from Leesa Pennell of BioLegend.
File: LEGENDplex-Attune-Setup.pdf