The UW Carbone Cancer Center Drug Development Core (DDC) supports world-class biomedical research by facilitating drug development in all therapeutic areas through the integration of high throughput screening, medicinal chemistry and pharmacology. These services are provided through the combined expertise and equipment of the Small Molecule Screening Facility (SMSF), the Medicinal Chemistry Center (MCC), and the Cancer Pharmacology Laboratory (CPL).
Cost effective high-throughput screening (HTS) generally from $0.10 to $1.00 per compound screened, depending on assay complexity. The DDC Small Molecule Screening Facility (SMSF) provides libraries of more than 500,000 small molecules, 290,000 biological fractions containing natural products, and numerous smaller specialized compound libraries. The SMSF also provides robotic liquid handling, plate readers, and other analysis instruments for a variety of cell-based and biochemical HTS assays in 96-, 384-, and 1536-well plate formats. Computational resources and expertise are provided for in silico screening of millions of compounds and for molecular modeling. The SMSF also offers specialized instruments including a Seahorse Bioanalyzer for cellular metabolism evaluation and a PerkinElmer Operetta High-Content Imaging System.
Facility Manager: Tom Polaske
The DDC Medicinal Chemistry provides synthesis of small molecules that have been identified as bioactive, development of lead and probe molecules for target identification, hit-to-lead optimization to improve drug-like properties, construction of libraries of small molecules, and large-scale preparative synthesis to support animal model studies. Evaluation of in vitro and in vivo pharmacology is provided through in-house assays and contracts maintained with external contract research organizations.
Facility Manager: Qiuhua Fan
The mission of the Cancer Pharmacology Lab is to advance cancer research by providing expertise; leadership; and bioanalytical assay development, validation and performance in support of the clinical, translational and laboratory research endeavors of UWCCC investigators.