Cancer Pharmacology Lab

What We Do

Services

Cancer Pharmacology Lab services include:

  • Sample Acquisition
  • Pharmacokinetic Analytical Assays
  • Pharmacodynamic Assays
  • Preclinical Analytic Services
W crest banner fluttering in the wind on a sunny day

Pharmacokinetics
Our instrumentation allows us to analyze a variety of matrices, including human and animal blood, plasma, serum, urine, breast and brain tissue.
We have validated HPLC and LC/MS assays for a variety of compounds, including ABT-888, Capecitabine, Cotinine, perillyl alcohol, polyamines, phenylephrine, Sorafenib, UAB30, and other compounds. We also offer method development services for the analysis of novel compounds.

Pharmacokinetics Example
Shown above is a histogram with diode array detector measurements of capecitabine metabolites (5’-Fluorouracil, 5’-Deoxy-5’Fluorocytidine, and 5’-Deoxy-5’Fluorouridine)

Pharmacodynamics
We offer a variety of pharmacodynamics analysis services, in coordination with other cores as needed.

Pharmacodynamics Example
Shown here is an analysis of rad51 foci, and MFI for γH2AX and PAR levels. Drug-treated cells (or untreated controls) were isolated from patient PBMCs and stained with antibodies to rad51, γH2AX, and PAR, along with a nuclear dye (DRAQ5). After staining, the cells were sorted by FlowImage in the Flow Lab, to determine MFI and to count individual foci.

Acknowledgments

Funding received from the UWCCC Cancer Center Support Grant (CCSG) for published research, including the use of UWCCC Shared Resources to generate or analyze data, or conduct clinical trials, needs to reference the UWCCC. See Acknowledging UWCCC in Publications, Posters, and Presentations.

View the UW Drug Development Core (DDC) page for more information on other UW–Madison laboratory facilities that provide state-of-the-art instrumentation and expert scientific staff.