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Technical Note describing method and steps for incorporating a bead standard into flow cytometry assays acquired on the Attune flow cytometer.

Assay standardization in flow cytometry improves reproducibility and reduces cytometer set-up time. For assays evaluating protein expression levels using MFI (median fluorescence intensity), this standardization is critical for reducing user set-up variation among replicates. For all flow assays, standardization expedites cytometer set-up and data analysis.
Updated 9/18/17

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Updated 9/18/17