Rigor & Reproducibility Seminar 121418
Flyer for Rigor & Reproducibility in Flow Cytometry Seminar 121418
Flow Cytometry Lab
Data Analysis with Alex 1 121318
Flyer for first in series of three interactive data analysis seminars presented by Alex Henkel
Flow Lab Data Analysis Office Hours
Flyer for Data Analysis Office Hours in the Flow Lab.
Attune Map
Table of common fluorochromes per channel, filter specs, and laser lines for Attune NxT flow cytometer.
File: WIMR-Attune-110918.pdfUpdate Grant Citation
Instructions for including Cancer Center Support Grant and Flow Lab S10 instrument grant acknowledgements to existing publications.
File: Updating-Grant-Acknowledgements.pdfUWFlowAriaJackMap032718
Optical bench map of BD FACSAria "Jack" Cell Sorter including common fluorochromes and default optical filters
File: UWFlowAriaJackMap032718.pdfUWFlowAriaJillMap032718
Optical Bench map of BD FACSAria Cell Sorter "Jill" listing common fluorochromes and default optical filters.
File: UWFlowAriaJillMap032718.pdfRainbow Bead Standardization – Attune
Technical Note describing method and steps for incorporating a bead standard into flow cytometry assays acquired on the Attune flow cytometer.
File: Rainbow-Standard-Tech-Note-Attune.pdfRegaining Keyboard Control After Diva Locks it Out
Periodically, Diva will stop accepting input from the keyboard. If the mouse is working but you cannot type to annotate your data, try the method explained the document to regain control.
Updated 9/18/17
Biosafety guidelines for cell sorting
Cell sorting is an aerosol-generating procedure, with greatly increases the risk of airway transmission of hazardous and infectious material. Aerosol incidents are not uncommon during normal sort procedures. These incidents can be caused by sample clogging and other disturbances that increase physical instability of the fragile single-cell stream.
Updated 9/18/17