It is important to consider the source of aggregation in your samples to properly address the issue. Adhesion can often be counteracted by removing divalent cations. However, the activity of DNAse requires divalent cations. Using EGTA instead of EDTA may allow magnesium ions to interact with DNAse while still partially mitigating adhesion.
Updated 9/18/17
File: Flow_TechNotes_Minimizing-Aggregates-in-Samples_20170918.pdf
This document covers the steps to add the mobile SIP to the home screen of your Apple device.
File: SIP_Apple.pdf
Dr. Mark Burkard's Precision Medicine Molecular Tumor Board presentation
File: WSP_Nov2016_revised.pptx
Flow Cytometry Laboratory MACSQuant10 Instrument Map.
File: UWCCC_Flow_MQ_Map.pdf
Designing and optimizing a multicolor flow experiment from the ground up takes a significant amount of front-end work to plan and optimize. This development time will save you time and precious sample in the long run!
Updated 9/18/17
File: Flow_TechNotes_Multicolor-flow-outline_20170918.pdf
Xiao et al.
A multifunctional unimolecular micelle made of a hyperbranched amphiphilic block copolymer was designed, synthesized, and characterized for cancer-targeted drug delivery and non-invasive positron emission tomography (PET) imaging in tumor-bearing mice. The hyperbranched amphiphilic block copolymer, Boltorn H40-poly(L-glutamate-hydrazone-doxorubicin)-b-poly(ethylene glycol) (i.e., H40-P(LG-Hyd-DOX)-b-PEG), was conjugated with cyclo(Arg-Gly-Asp-D-Phe-Cys) peptides (cRGD, for integrin avb3 targeting) and macrocyclic chelators (1,4,7-triazacyclononane-N, N’, N’’-triacetic acid [NOTA], for 64Cu-labeling and PET imaging) (i.e., H40-P(LG-Hyd-DOX)-b-PEG-OCH3/cRGD/NOTA, also referred to as H40-DOX-cRGD). The anti-cancer drug, doxorubicin (DOX) was covalently conjugated onto the hydrophobic segments of the amphiphilic block copolymer arms (i.e., PLG) via a pH-labile hydrazone linkage to enable pH-controlled drug release. The unimolecular micelles exhibited a uniform size distribution and pH-sensitive drug release behavior. cRGD-conjugated unimolecular micelles (i.e., H40-DOX-cRGD) exhibited a much higher cellular uptake in U87MG human glioblastoma cells due to integrin avb3-mediated endocytosis than non-targeted unimolecular micelles (i.e., H40-DOX), thereby leading to a significantly higher cytotoxicity. In U87MG tumor-bearing mice, H40-DOX-cRGD-64Cu also exhibited a much higher level of tumor accumulation than H40-DOX-64Cu, measured by non-invasive PET imaging and confirmed by biodistribution studies and ex vivo fluorescence imaging.We believe that unimolecular micelles formed by hyperbranched amphiphilic block copolymers that synergistically integrate passive
and active tumor-targeting abilities with pH-controlled drug release and PET imaging capabilities provide the basis for future cancer theranostics.
File: Xiao_et_al_Multifunctional_unimolecular_micelles_for_cancer.pdf
This is a sample document
File: Rigor-and-Reproducibility-in-Flow.pdf
Sample NDA
File: Non-Disclosure_Agreement_TEMPLATE.pdf
Sample Non-Discolosure Agreement for Outside-User Collaborations
BCRAN Meeting Agenda for 11/11/2015
File: BCRAN-nov-11.pdf
OTRS Agenda for September 14, 2016
File: 20160914-Meeting-Agenda.pdf