Titration allows you to determine the amount of antibody that gives you the best separation of populations in your samples, and the best measure of expression levels. Too little antibody means the cells expressing the marker of interest do not stain as brightly as they could, and may not separate adequately from the negative cells. Too much antibody can increase non-specific binding, which increases the spread and background of the negative population. Both situations result in lower resolution of the measurement.
File: Flow_TechNotes_Antibody-Titrations_20170918.pdf
TMA slide reqeust for services.
File: TMA_Slide_Request_for_Services.pdf
Print out this flyer and put it on your door to let UWCCC staff know to stop in to say hi and grab some treats!
File: TrickorTreat-1.pdf
Instructions for including Cancer Center Support Grant and Flow Lab S10 instrument grant acknowledgements to existing publications.
File: Updating-Grant-Acknowledgements.pdf
UW Customers Paying with Non-UW Funding in iLab Customer Guide
File: UW-Customers-Paying-with-Non-UW-Funding-in-iLab-Customer-Guide.pdf
UWCCC Administrative Letterhead Template
File: Administration_letterhead_2017.doc
Listing of Grand Rounds speakers and topics for December 2017
File: UWCCC-Grand-Rounds-December-2017-Notice2.pdf
Listing of speakers and topics for January 2018.
Grand Rounds schedule for November 2017. To view the Grand Rounds schedule and watch videos of past Grand Rounds, please visit the UWCCC Grand Rounds webpage: https://cancer.wisc.edu/research/education/grand-rounds/
File: UWCCC-Grand-Rounds-November-2017-Notice.pdf