Compensation beads offer a variety of benefits. The most obvious of these is that they allow you to save more of your cells for your experimental conditions rather than controls. Additionally, compensation beads stain more brightly and uniformly than cells, allowing for easy compensation of colors that may be only on rare events in the cells sample. Importantly, compensation beads can be used with the same antibodies you use for your experiment ensuring a perfect fluorochrome match.
Updated 9/18/17
File: Flow_TechNotes_Compensation-Beads-Tech-Note_20170918.pdf
Titration allows you to determine the amount of antibody that gives you the best separation of populations in your samples, and the best measure of expression levels. Too little antibody means the cells expressing the marker of interest do not stain as brightly as they could, and may not separate adequately from the negative cells. Too much antibody can increase non-specific binding, which increases the spread and background of the negative population. Both situations result in lower resolution of the measurement.
File: Flow_TechNotes_Antibody-Titrations_20170918.pdf
Guidance on how to add flow cytometry and cell sorting experiments to Bio-ARROW protocols
File: UWCCC_Flow_Bio_ARROW.pdf
Flow Cytometry Laboratory BD FACS Calibur instrument map
File: UWCCC_Flow_Calibur_Map.pdf
Flow Cytometry Laboratory ImageStream Instrument Map
File: UWCCC_Flow_ImageStream_Map.pdf
Flow Cytometry Laboratory 5 Laser BD LSRII Instrument Map
File: UWCCC_Flow_LSR_II_Map_06102015.pdf
Flow Cytometry Laboratory MACSQuant10 Instrument Map.
File: UWCCC_Flow_MQ_Map.pdf