DDC – Cancer Pharmacology Laboratory

Major instrumentation for analytical assays are HPLC, LCMS and LCMSMS. These assays may be performed in any substrate including plasma, serum, urine, whole blood, erythrocytes or tissue.

In addition to the currently available assays listed below, the Cancer Pharmacology Core will develop, validate and run almost any assay needed in your research.

The Cancer Pharmacology Lab will validate and run a variety of PD assays.

For example:

• Various Cytokines
• HIF-1a
• Prostate cancer panel: IGF1, IGFBP3, Testosterone, PSA, Urine 2-Hydroxyestrogen and 16alpha-hydroxyestrone
• TGF1, TGF2
• VEGF pathway (VEGF, bFGF, MIP1, VEGFR1, VEGFR2)

The Cancer Pharmacology Lab provides analytical chemistry services using HPLC or LC-MS to quantify small molecule drugs in user provided biological samples including blood, urine or tissue.

• Advice on appropriate experimental design
• Problem definition and statistical methodology
• Definition of study endpoints
• Sample size requirements
• Definition of data items required
• Determination of randomization and stratification

If necessary and for clinical and population-based studies:

• Selection of study subjects and appropriate control groups
• Recruitment strategies
• Availability of data from related studies
• Design of case report forms

Kit Drop Off

Please fill out the Kit Drop Off form indicating that you have dropped off a kit and include the study number and the intended use date.

Lab access is restricted to primary lab staff.

UH: Kit drop-off will be on cart outside lab of K4/559 before 4:30pm.

EMC: Kits should be placed on the shelf labeled with the appropriate day.

All kits should be delivered 2-7 days ahead of visit.
Staff must be notified of any specimens being dropped off by calling 263-5369 at UH, 914-0501 at EMC, or ahead of time by emailing cplabsas@medicine.wisc.edu for either site (please specify which).

Phamacokinetic Assays 

Pharmacodynamic Assays 

Preclinical Analytic Services 

•   Pharmacometric modeling: Non-compartmental and Population PK 
•   Determination of appropriate statistical methodology 
•   Production of final study reports including graphs and tables or other summaries suitable for presentation and publication 
•   Interpretation of statistical results, appropriate documentation and relevant literature

The Cancer Pharmacology Lab offers the following equipment for use by investigators. View charges for associated fees, and log into iLab to schedule equipment.

• Shimadzu Prominence HPLC system with DAD, fluorescence, and UV detectors
• NanodropND-1000 Spectrophotometer
• SpectraMax M3 Micro-Plate Reader

Any published research which received funding from the UWCCC Cancer Center Support Grant (CCSG), including the use of UWCCC Shared Resources to generate or analyze data or conduct clinical trials, must acknowledge the UWCCC. See Acknowledging UWCCC in Publications, Posters, and Presentations. Please acknowledge “UWCCC Circulating Biomarker Core” for any work supported by the CBC.  Thank you!

Sample Acknowledgment

“The author(s) thank the University of Wisconsin Carbone Cancer Center DDC Cancer Pharmacology Laboratory, supported by P30 CA014520, for use of its facilities and services.”

The Cancer Pharmacology Lab is proud to offer its services at competitive rates.

You can now pay by credit card using our Online Bill Pay option

Activation and Maintenance Fees

The activation and maintenance fees are used for Protocol-specific training, computer and OnCore database support, equipment maintenance, quality assurance audits, office supplies, dry ice, and on-site meeting attendance associated with the study review, budgeting, activation and maintenance by CPL personnel.

Consumable Supplies

All consumable supplies not provided will be charged at actual cost.

Shipping

In all cases the Sponsor or research group must pay shipping charges. Groups using CPL under a Fee for Service Agreement will negotiate shipping charges on a case-by-case basis.