Glasswash and Scientific Instrumentation Facilities
The Glass Washing and Scientific Instrumentation facilities provide centralized services to support laboratories in the UW Carbone Cancer Center. The Glass Washing Facility provides cleaning and sterilizing laboratory glassware and media, and lab coat laundering. The Scientific Instrumentation Facility support includes the procurement and maintenance of shared laboratory equipment and the relocation and coordination of laboratory equipment, facilities and related infrastructure.
Leadership
Scientific Instrumentation Facility Manager: WIMR and CSC
Scientific Instrumentation Facility Manager: McArdle
Glasswash Facility Manager
Scientific Instrumentation Facility
The Scientific Instrumentation Facility (SIF) provides centralized support to UW Carbone Cancer Center (UWCCC) Shared Resources, cancer center members and non-members involved in laboratory, translational and clinical research. Support includes the procurement and maintenance of shared equipment; relocation and coordination of laboratory equipment, facilities and related infrastructure; and solutions to everyday problems.
Glassware Washing and Sterilization
Services
- High Temperature glassware washing and acid cleaning
- Steam sterilization (autoclave)
- Dry Sterilization: bake at 200-200+C for 6 hours
- RNAse deactivation via dry sterilization
- Acid cleaning and dry sterilization of glass pipettes
- Pipette tip sterilization
- Desiccant Reactivation via baking at 220C for 6-18 hours
- Washing and Sterilization of incubator shelves
- Sourcing and Autoclaving ultrapure water at 18.2 Megaohms
Glassware
| Service | Cost |
|---|---|
| Wash | $20.00/load* |
| Autoclave | $20.00/load* |
| Bake (constant temp. oven) | $20.00/load* |
Pipettes
| Service | Cost |
|---|---|
| Glass Pipettes | $20.00/can |
| Pasteur Pipettes | $20.00/can |
| Pipette Tips | $20.00/load* |
- Labs should email Rob to set up a consultation and get set up with the Glass Washing services and incorporated into the rounds.
- Glass Washing and Sterilization
- As labs use equipment, they empty and rinse glassware and place them on the lower shelves of the wire rack that most labs have in the LER.
- Once a lab decides that it is time to have equipment washed and sterilized, they email Rob. In that email, they request a pick-up and include the PI and location (room #). Rob will pick up, wash, sterilize, and return the equipment, usually within 24 hours.
- Lab Coat Laundering
- When a UWCCC member lab or an individual lab needs lab coats laundered, they place them in the laundry bag outside of 4112 in WIMR I or outside of K4/566.
- Once the coats are in the bag, please fill out the form hanging above the laundry bag. It asks for the date, name, lab/PI, room number, and the number of lab coats placed in the bag.
- Lab coats are picked up and returned on Mondays and Thursdays.
- Washing
- We do expect labs to rinse glassware before submitting to glass-wash services.
- This washer first uses a detergent in a high temperature wash. Then it uses an acid rinse to effectively neutralize alkaline detergent residue, remove mineral deposits and protein residues, and prevent contamination.
- Finally, the washers go through a high temp drying cycle. This process takes about 90 minutes in total.
- Sterilization
- Autoclaves are used to sterilize any glass that includes a screw-on lid. Using high temperatures, steam, and high pressure, is effective in killing microorganisms and spores.
- Our Sterilization Oven is used for open glass vessels such as beakers, cylinders, and pipettes. Glass gets baked at 200C for six hours. This sterilization process is also used to deactivate glass that may contain RNAse.
- The Sterilization Oven is also used to recharge desiccant. We bake it at 220C for 6-18 hours.