Rigor and Reproducibility – Get Involved!

Minimum Set of Research Flow Cytometry Standards (MiSET RFC Standards) – where publishing reproducible flow cytometry data starts


MiSET RFC Standards is a recently launched ISAC Marylou Ingram Scholar-led initiative that aims to integrate quality-defining parameters of a flow cytometry experiment into peer-review and publication procedures. Over a three-year period, MiSET RFC Standards will assemble a universally applicable, evidence-based checklist that helps to quickly and confidently assess the overall quality of flow cytometry data.


Be part of the first major strategic goal of MiSET RFC Standards by participating in our SURVEY! Your answers will help us understand the current approach to flow cytometry practices. Click here to answer a few questions (max. 10 minutes required) https://forms.gle/XdX4HfW6sebxAo5Z9.


The survey will be followed by EXPERT EVALUATION to compose a preliminary MiSET RFC consensus checklist highlighting the minimum requirements for an entire flow cytometry experiment. We will test the suitability of the preliminary MiSET RFC consensus checklist for real world biomedical research settings through USER ACCEPTANCE TESTING. The FINAL PRODUCT is envisioned to be a benchmark for any research flow cytometry experiment that is an integral part of manuscript and grant submissions across biomedical fields that rely on the use of flow cytometry.

Do you want to learn more about MiSET RFC? The MiSET RFC mission statement, highlighting the strategic goals and milestones, is published in Cytometry A – click here to check it out https://www.ncbi.nlm.nih.gov/pubmed/31769204. More information can also be found on our website https://misetrfc.com/.